plasma il 6 concentration  (Boster Bio)

Journal: Experimental physiology

Article Title: Increased skeletal muscle mitochondrial free radical production in peripheral arterial disease despite preserved mitochondrial respiratory capacity

doi: 10.1113/EP086905

Figure Lengend Snippet: Representative Western Blot images are presented for IL-6 and 4-HNE (panels A and B, respectively). Panel A, HC (n = 9) and PAD (n = 7); Panels B and C, HC (n = 9) and PAD (n = 7); Panel D, HC (n = 12) and PAD (n = 10). Data presented as mean ± SEM. *Significant difference between groups, (p < 0.05).

Article Snippet: *Significant correlation, ( p < 0.05). table ft1 table-wrap mode="anchored" t5 Table 5: caption a7 Relationship r P Value Inflammation Plasma IL-6/Intramuscular PC (nmol/mg protein) 0.64 0.01 Plasma IL-6/Plasma MDA (nM) 0.55 0.02 ABI ABI/Intramuscular PC (nmol/mg protein) −0.80 0.001 ABI/Intramuscular 4-HNE (fold beta actin) −0.66 0.006 ABI/Intramuscular IL-6 (fold beta actin) −0.70 0.003 ABI/Mitochondrial-derived ROS production (U/mg tissue) −0.55 0.03 ABI/Plasma TNF-α Concentrations (pg/ml) −0.56 0.01 ABI/Plasma IL-6 Concentrations (pg/ml) −0.54 0.02 Open in a separate window Notes: TNF-α = tumor necrosis factor alpha, IL-6 = interleukin 6, CRP = C-reactive protein, MDA = malondialdehyde, PC = protein carbonyls, r = Pearson correlation.

Techniques: Western Blot

Journal: Experimental physiology

Article Title: Increased skeletal muscle mitochondrial free radical production in peripheral arterial disease despite preserved mitochondrial respiratory capacity

doi: 10.1113/EP086905

Figure Lengend Snippet: Evidence of a relationship between mitochondrial state 2 respiration and intramuscular 4-HNE (panel A); plasma concentrations of TNF-α and intramuscular PC (panel B); plasma concentrations of IL-6 and intramuscular 4-HNE (panel C); and mitochondrial ROS production and intramuscular protein carbonyls (panel D). HC (n = 9) and PAD (n = 7). *Significant correlation, (p < 0.05).

Article Snippet: *Significant correlation, ( p < 0.05). table ft1 table-wrap mode="anchored" t5 Table 5: caption a7 Relationship r P Value Inflammation Plasma IL-6/Intramuscular PC (nmol/mg protein) 0.64 0.01 Plasma IL-6/Plasma MDA (nM) 0.55 0.02 ABI ABI/Intramuscular PC (nmol/mg protein) −0.80 0.001 ABI/Intramuscular 4-HNE (fold beta actin) −0.66 0.006 ABI/Intramuscular IL-6 (fold beta actin) −0.70 0.003 ABI/Mitochondrial-derived ROS production (U/mg tissue) −0.55 0.03 ABI/Plasma TNF-α Concentrations (pg/ml) −0.56 0.01 ABI/Plasma IL-6 Concentrations (pg/ml) −0.54 0.02 Open in a separate window Notes: TNF-α = tumor necrosis factor alpha, IL-6 = interleukin 6, CRP = C-reactive protein, MDA = malondialdehyde, PC = protein carbonyls, r = Pearson correlation.

Techniques:

Journal: Experimental physiology

Article Title: Increased skeletal muscle mitochondrial free radical production in peripheral arterial disease despite preserved mitochondrial respiratory capacity

doi: 10.1113/EP086905

Figure Lengend Snippet: Correlations between Inflammation, Free Radical Production, and Oxidative Stress

Article Snippet: *Significant correlation, ( p < 0.05). table ft1 table-wrap mode="anchored" t5 Table 5: caption a7 Relationship r P Value Inflammation Plasma IL-6/Intramuscular PC (nmol/mg protein) 0.64 0.01 Plasma IL-6/Plasma MDA (nM) 0.55 0.02 ABI ABI/Intramuscular PC (nmol/mg protein) −0.80 0.001 ABI/Intramuscular 4-HNE (fold beta actin) −0.66 0.006 ABI/Intramuscular IL-6 (fold beta actin) −0.70 0.003 ABI/Mitochondrial-derived ROS production (U/mg tissue) −0.55 0.03 ABI/Plasma TNF-α Concentrations (pg/ml) −0.56 0.01 ABI/Plasma IL-6 Concentrations (pg/ml) −0.54 0.02 Open in a separate window Notes: TNF-α = tumor necrosis factor alpha, IL-6 = interleukin 6, CRP = C-reactive protein, MDA = malondialdehyde, PC = protein carbonyls, r = Pearson correlation.

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: Lyophilized powder of mesenchymal stem cell supernatant attenuates acute lung injury through the IL-6–p-STAT3–p63–JAG2 pathway

doi: 10.1186/s13287-021-02276-y

Figure Lengend Snippet: MSC SLP alleviated pro-inflammatory cytokine production induced by BLM. a MSD electro-chemiluminescence assays. b , c IL-6 concentrations in BALF and plasma samples were measured by ELISA. d IL-1β concentrations in BALFs were detected by ELISA. N = 6–8 in each group. The data shown are presented as the mean ± SD, and statistical differences were assessed by one-way ANOVA. * P < 0.05; *** P < 0.001

Article Snippet: Then, plasma IL-6 concentrations and BALF IL-6 and IL-1β concentrations were measured using corresponding enzyme-linked immunosorbent assay (ELISA) kits from eBioscience (San Diego, CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Stem Cell Research & Therapy

Article Title: Lyophilized powder of mesenchymal stem cell supernatant attenuates acute lung injury through the IL-6–p-STAT3–p63–JAG2 pathway

doi: 10.1186/s13287-021-02276-y

Figure Lengend Snippet: MSC SLP inhibited STAT3 phosphorylation, and rh IL-6 enhanced STAT3 phosphorylation to reduce p63 expression. a The levels of p-STAT3, STAT3, and β-actin proteins were measured by western blotting. b Quantitative analysis of p-STAT3 expression. c The concentrations of rh IL-6 in the lungs on days 1 and 2 after rh IL-6 administration. d The protein levels of p-STAT3, STAT3, p63, and β-actin were measured by western blotting. e Quantitative analysis of p-STAT3 expression. f p63 mRNA-expression levels were measured by RT-qPCR. N = 6–8 in each group. The data shown are presented as the mean ± SD, and statistical differences were assessed by one-way ANOVA. ** P < 0.01; *** P < 0.001

Article Snippet: Then, plasma IL-6 concentrations and BALF IL-6 and IL-1β concentrations were measured using corresponding enzyme-linked immunosorbent assay (ELISA) kits from eBioscience (San Diego, CA, USA).

Techniques: Expressing, Western Blot, Quantitative RT-PCR

Journal: Stem Cell Research & Therapy

Article Title: Lyophilized powder of mesenchymal stem cell supernatant attenuates acute lung injury through the IL-6–p-STAT3–p63–JAG2 pathway

doi: 10.1186/s13287-021-02276-y

Figure Lengend Snippet: MSC SLP increased JAG2 expression by activating p63. a JAG2 mRNA-expression levels in a mouse model of ALI. b The protein-expression levels of JAG2 and β-actin in a mouse model of ALI. c Quantitative analysis of JAG2 expression. d The protein-expression levels of JAG2 and β-actin in mice treated with IL-6 ± SLP. e Quantitative analysis of JAG2 expression. f Schematic model of how MSC SLP attenuates BLM-induced ALI. N = 6–8 in each group. The data shown are presented as the mean ± SD, and statistical differences were assessed by one-way ANOVA. ** P < 0.01; *** P < 0.001

Article Snippet: Then, plasma IL-6 concentrations and BALF IL-6 and IL-1β concentrations were measured using corresponding enzyme-linked immunosorbent assay (ELISA) kits from eBioscience (San Diego, CA, USA).

Techniques: Expressing